Protein A Ceramic HyperD F Affinity Chromatography Sorbent Laboratory Scale Volumes. 25 mL. Particle Size: 50 um. Dynamic Binding Capacity for Human IgG (10% breakthrough 100 cm/h): 30 mg/mL. Ligand: Recombinant Protein A. Immobilized Protein A: 4 - 5 mg/mL of sorbent.