BBL AK Agar 2 (Sporulating Agar), 500g

AK Agar #2 (Sporulating Agar) is a culture medium for the preparation of spore suspensions for use in procedures for the detection of antibiotic residues in milk and dairy products.

AK Agar #2 was devised by Arret and Kirshbaum for specificuse in the production of spores of Bacillus subtilis ATCC™ 6633 for use in the Penicillin Milk Test procedure. This medium was formerly specified in the spore preparation phase of the American Public Health Association disc assay procedure for the detection of sulfa drugs and antibiotics in milk. 

Principles of the Procedure
The peptones and beef extract are sources of nitrogen, sulfur, amino acids and essential trace ingredients. Yeast extract is a rich source of B vitamins. Dextrose is an energy source for bacterial replication. Manganous sulfate plays an important role in the sporulation process.

Formula
BBL™ AK Agar #2
Approximate Formula* Per Liter
Pancreatic Digest of Gelatin ....................................... 6.0 g
Pancreatic Digest of Casein ........................................ 4.0 g
Yeast Extract .............................................................. 3.0 g
Beef Extract ................................................................ 1.5 g
Dextrose ..................................................................... 1.0 g
Agar ......................................................................... 15.0 g
Manganous Sulfate .................................................... 0.3 g
*Adjusted and/or supplemented as required to meet performance criteria.