BD Difco M9 Minimal Salts 5x, 500g

BD Difco M9 Minimal Salts 5x, 500g - BD Difco Catalog 248510

Intended Use
M9 Minimal Salts, 5× is used in preparing M9 Minimal
Medium which is used for cultivating recombinant strains of
Escherichia coli.

Summary and Explanation
M9 Minimal Salts, 5× is a 5× concentrate that is diluted to
a 1× concentration and supplemented with an appropriate
carbon and energy source, such as dextrose, to provide a
minimal, chemically defined medium. The medium will support
the growth of “wild-type” strains of E. coli. M9 Minimal
Salts is useful for maintaining positive selection pressure on
plasmids coding for the ability to produce essential substances
such as amino acids or vitamins. M9 Minimal Medium is also
used to maintain stocks of F´-containing bacteria for use with
M13. The medium can be supplemented with specific amino
acids or other metabolites, allowing for selection of specific
auxotrophs.

Principles of the Procedure
Sodium phosphate and potassium phosphate are present as
buffering agents. Ammonium chloride is a source of nitrogen for
cellular systems. Sodium chloride provides essential ions. Glucose
may be added as a source of carbohydrate. Supplementing the
medium with magnesium and calcium increases the growth of
recombinants.

Formula
Difco™ M9 Minimal Salts, 5×
Approximate Formula* Per Liter
Disodium Phosphate (anhydrous)............................... 33.9 g
Monopotassium Phosphate........................................ 15.0 g
Sodium Chloride.......................................................... 2.5 g
Ammonium Chloride.................................................... 5.0 g
*Adjusted and/or supplemented as required to meet performance criteria.
Directions for Preparation from
Dehydrated Product
1. Dissolve 56.4 g of the powder in 1 L of purified water.
2. Autoclave at 121°C for 15 minutes.
3. To prepare M9 Minimal Salts Medium, add 200 mL sterile
M9 Minimal Salts, 5× to 750 mL sterile purified water cooled
to 45-50°C, adjusting the final volume to 1 liter.