MacConkey II Agar with Sorbitol, BBL, 500g

MacConkey II Agar with Sorbitol, BBL 299769, 500g

Principles of the Procedure: Use of MacConkey II Agar with Sorbitol

MacConkey Sorbitol Agar and MacConkey II Agar with Sorbitol, modified MacConkey agars using sorbitol instead of lactose, are only slightly selective, since the concentration of bile salts, which inhibits gram-positive microorganisms, is low in comparison with other enteric plating media. Crystal violet also is included in the medium to inhibit the growth of gram-positive bacteria, especially enterococci and staphylococci.
MacConkey II Agar with Sorbitol is also formulated to reduce swarming of Proteus species. Differentiation of enteric microorganisms is achieved by the combination of sorbitol and the neutral red indicator. Colorless or pink to red colonies are produced depending upon the ability of the isolate to ferment the carbohydrate sorbitol.

BBL™ MacConkey II Agar with Sorbitol
Approximate Formula* Per Liter
Pancreatic Digest of Gelatin....................................... 17.0 g
Pancreatic Digest of Casein.......................................... 1.5 g
Peptic Digest of Animal Tissue...................................... 1.5 g
D-Sorbitol.................................................................. 10.0 g
Bile Salts...................................................................... 1.5 g
Sodium Chloride.......................................................... 5.0 g
Agar.......................................................................... 13.5 g
Neutral Red.................................................................. 0.03 g
Crystal Violet............................................................... 1.0 mg

*Adjusted and/or supplemented as required to meet performance criteria.