BD BBL™ DrySlide™ Nitrocefin, Pk of 25

BD BBL™ DrySlide™ Nitrocefin, Pack of 25

INTENDED USE

BBL™ DrySlide™ Nitrocefin is used for detecting β-lactamase production by bacteria.

SUMMARY AND EXPLANATION The chromogenic cephalosporin beta-lactamase test is a qualitative procedure for detecting the production of beta-lactamase by bacteria. The reaction is based on the production of a colored compound when the substrate, nitrocefin, is exposed to a β-lactamase-producing culture. The enzyme, beta-lactamase, originally described by Abraham and Chain, is produced by various organisms and is a mechanism of their resistance to penicillins and cephalosporins. Test methods used to detect β-lactamase include iodometric, acidimetric and chromogenic cephalosporin procedures. Some staphylococci may require induction (exposure to a β-lactam agent) to increase production of β-lactamase to detectable levels. According to standard references, any negative β-lactamase test result from a non-induced stapylococcal isolate, despite the test method, should be confirmed by induction of the isolate and repeat testing. BBL DrySlide Nitrocefin employs nitrocefin, a cephalosporin compound first described by Glaxo Research (Middlesex, England), in the chromogenic cephalosporin test methodology