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Gaspak EZ Anaerobe System BBL 260683 Maximize

Gaspak EZ Anaerobe System BBL 260683

Gaspak EZ Anaerobe System BD BBL 260683. Sold as a pack of 20

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BD-260683

$93.56

Gaspak EZ Anaerobe System, Pk of 20 BBL BD 260683

INTENDED USE
The GasPak™ EZ Gas Generating Pouch Systems are single-use systems that produce atmospheres suitable to support the primary isolation and cultivation of anaerobic, microaerophilic, or capnophilic bacteria by use of gas generating sachets inside single-use resealable pouches.
SUMMARY AND EXPLANATION
In 1965, Brewer and Allgeier introduced a disposable hydrogen generator envelope, which was later modified to include carbon dioxide generation and an internal catalyst. The GasPak EZ Gas Generating Pouch Systems offer bio-performance and reliability without the need for a catalyst or an activation procedure. The GasPak EZ Gas Generating Sachets contain all the ingredients needed to create a specific atmosphere for specimen incubation. The sachet and specimens are placed in the GasPak EZ resealable pouch. The pouch is sealed and incubated.

PRINCIPLES OF THE PROCEDURE
The GasPak EZ Gas Generating Sachet consists of a reagent sachet containing inorganic carbonate, activated carbon, ascorbic acid and water. When the sachet is removed from the outer wrapper, the sachet becomes activated by exposure to air. The activated reagent sachet and specimens are placed in the resealable pouch and sealed. The sachet rapidly reduces the oxygen concentration within the pouch. At the same time, inorganic carbonate produces carbon dioxide.

PROCEDURE
Materials Provided: GasPak EZ Gas Generating Pouch System. Each system requires the use of a resealable pouch and sachet.
Materials Required But Not Provided: Ancillary culture media, reagents, quality control organisms and laboratory equipment as required for this procedure.

Test Procedure:
1. Place the inoculated plates inside the resealable pouch. The GasPak EZ Campy Pouch System requires at least 2 Petri dishes and will hold up to 4 petri dishes. The GasPak EZ CO2 Pouch System and the GasPak EZ Anaerobe Pouch System with Indicator can be used for 1-4 Petri dishes. Note: For optimum growth using the GasPak EZ Campy Pouch System, place a paper towel or cotton ball moistened with 5mL of water inside the pouch.

2. One GasPak EZ Pouch System Sachet is used in each resealable pouch. Remove the GasPak EZ Pouch System Sachet from the carton. Remove the outer foil packaging.

3. Place the activated sachet in the GasPak EZ resealable pouch with the plates. The sachet should be placed between the plates and the pouch. If using the GasPak EZ Anaerobe Pouch System with Indicator, there is no need to add a separate anaerobic indicator.

4. Close the pouch by pressing the zipper part of the pouch together.

5. Incubate the GasPak EZ Pouch System at a temperature appropriate for the organism being cultured, but not to exceed 45ºC.

6. After incubation, open the pouch, remove the plates and dispose of the GasPak EZ Pouch System Sachet, indicator and pouch in the appropriate manner.